Our project is placed into The Collaborative Research Centre (CRC) 973 “Priming and Memory of Organismic Responses to Stress“ (http://www.sfb973.de/). Our project will elucidate how the sequential exposure to antimicrobials impacts on stress responses and fitness of the model bacterium Escherichia coli. We will use a suite of antimicrobial peptides and synthetic antibiotics and relate this to our understanding of immune effectors in a model insect during a persistent infection. We will then investigate how priming by the first stressor leads to an altered response against a second stressor studying growth rate in vitro and virulence in an insect model as performance indicators. By RNA-seq, including mRNAs and small-RNAs, we will try to determined the molecular basis of the altered response during priming events in bacteria.

Participants:

Jens Rolff – PI (FU Berlin)
Alexandro Rodriguez-Rojas – Postdoc (FU Berlin)

Involved people:

Susan Mbedi (lab work library preparation)
Kirsten Richter (lab work library preparation)

Applied methods:

RNAseq (sequencing mRNAs ans small-RNAs)

Status at BeGenDiv:

ongoing

Funding:

CRC973 project funded by DFG (Deutsche Forschungsgemeinschaft)

Duration:

4 years

Links:

http://www.sfb973.de/research/Project_C5/index.html

Status at BeGenDiv:

Ongoing

Rodríuez-Rojas A, Makarova O, Rolff J (2014)

Antimicrobials, Stress and Mutagenesis.

Picture of E.coli was taken by Alexandro Rodríguez-Rojas.